Ethanol Productivity of Ethanol-Tolerant Mutant Strain Pichia kudriavzevii R-T3 in Monoculture and Co-culture Fermentation with Saccharomyces cerevisiae

We previously developed ethanol-tolerant P. kudriavzevii R-T3 (P.ku R-T3) mutant from its parental strain P.ku R-WT by evolutionary adaptation method. Hence, we further analyze the ethanol productivity of particular isolates in a monoculture and co-culture with industrial yeast Saccharomyces cerevisiae BY4741 at various inoculum ratio. Based on spot assay, R3 showed better cell viability under 10% stress than wild type, potentially due to high expression level PKINO1 gene involved synthesis inositol. In fermentation, S. could use glucose, while mixed glucose xylose as carbon sources for fermentation. performed most potential kinetics parameters, including highest production (10.10 g/L), (0.21 g/L/h), fermentation efficiency (84.36%). Upscaling ten times resulted higher production. However, substrate utilization rate did not indicate an increase Indeed, low but produced cerevisiae, much 21-31%, depending initial inoculum. Interestingly, substantially produce technique. About 30% reduction was found co-culturing alone. Taken together, remains promising technique that cerevisiae.